BIOSENSOR FOR ALT MEASUREMENT

نویسندگان

چکیده

In this work, an amperometric biosensor to determine ALT, which will be part of a system for simultaneous measurement AST and ALT their ratios, was developed. A platinum disk electrode used as the electrochemical transducer. To form bioselective element, mixture based on glutamate oxidase applied surface universal method measuring activity using developed proposed described. During study, optimal concentrations substrates (α-ketoglutarate - 50 μM, alanine 4 mM) coenzyme (50 mkM PLP) were selected. The analytical characteristics also investigated, namely, sensitivity, linear range, minimum limit determination, selectivity, stability, etc. It shown fundamental possibility measure content in real samples.

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ژورنال

عنوان ژورنال: Sensorna elektronìka ì mìkrosistemnì tehnologìï

سال: 2022

ISSN: ['2415-3508', '1815-7459']

DOI: https://doi.org/10.18524/1815-7459.2022.1/2.239789